In both developed and under-developed countries, parasitic nematodes’ eggs that infect grazing ruminants are a major economic threat 3, 4. ![]() ![]() This infection is predominantly present in resource-limited settings located in tropical and subtropical countries of the world 2. The soil-transmitted helminths (parasitic worms) are causative agents of helminthiasis disease that infect 2.5 billion people across the globe 1. This non-invasive imaging technique could lead to better understanding of these species and may assist in early control of diseases. Using confocal microscopy, we investigate the autofluorescence properties of five species of nematode eggs and observe clear differences between genus and for the first time their species in sludge samples. In this study, we demonstrate that non-invasive real-time identification of eggs is possible based on their intrinsic fluorescence. However, these techniques are not consistent and often laborious and time-consuming. Numerous methods exist for nematode identification, from a morphological-based approach to high throughput sequencing technology. Identifying eggs of helminths from wastewater and sludge is important from a public health perspective to minimize the spread of Ascaris infections. However, their eggs, particularly their species Ascaris lumbricoides and Ascaris suum cannot be identified from each other. Adult parasitic nematode worms can be distinguished based on their size and morphology. Nematode eggs are pervasive pathogens that infect billions of people and livestock every year.
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